Mass Appeal: lessons learnt from large-scale mass spectrometry-based metabolomics
Metabolomics is a growing discipline that allows the analysis of the thousands of structural different small molecules found within a biological system. These metabolites can be measured using a variety of different analytical approaches and at Manchester we have developed GC-MS and LC-MS (Dunn et al., 2011). I shall provide an overview of metabolomics and lessons learnt from of our large-scale human serum metabolome project (www.husermet.org) where we profiled 1200 healthy individuals (Dunn et al., 2015). Using these protocols we then went on to profile another ~1200 ageing individuals and identified key Metabolic dysregulation which were drivers behind human frailty (Rattray N.J. et al., under review).
Key aspects for discussion will be based around a SWOT analysis of the field.
Dunn, W.B., Broadhurst, D., Begley, P., Zelena, E., Francis-McIntyre, S., Anderson, N., Brown, M., Knowles, J.D., Halsall, A., Haselden, J.N., Nicholls, A.W., Wilson, I.D., The Husermet consortium, Kell, D.B. & Goodacre, R. (2011) Procedures for large-scale metabolic profiling of serum and plasma using gas chromatography and liquid chromatography coupled to mass spectrometry. Nature Protocols 6, 1060-1083.
Dunn, W.B., Lin, W., Broadhurst, D., Begley, P., Brown, M., Zelena, E., Vaughan, A.A., Halsall, A., Harding, N., Knowles, J.D., Francis-McIntyre, S., Tseng, A., Ellis, D.I., O’Hagan, S., Aarons, G., Benjamin, B., Chew-Graham, S., Moseley, C., Potter, P., Winder, C.L., Potts, C., Thornton, P., McWhirter, C., Zubair, M., Pan, M., Burns, A., Cruickshank, J.K., Jayson, G.C., Purandare, N., Wu, F.C.W., Finn, J.D., Haselden, J.N., Nicholls, A.W., Wilson, I. D., Goodacre, R. & Kell, D.B. (2015) Molecular phenotyping of a UK population: defining the human serum metabolome. Metabolomics 11, 9-26.